Biotinylated Human Claudin 6 Nanodisc
Catalog No: CLDN6-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Claudin 6 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val220 (UniProt accession: P56747).
Product Details
- Molecular Aliases
- Claudin-6; Skullin; CLDN6; Claudin6; Skullin 2
- Protein Length
- Met1-Val220
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 36.20 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human Claudin 6 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human Claudin 6 Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-Claudin6 Antibody, mFc Tag with an affinity constant of 0.35 nM as determined in BLI assay.
Background
Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.
References
- Sugimoto K, Ichikawa-Tomikawa N, Kashiwagi K, Endo C, Tanaka S, Sawada N, Watabe T, Higashi T, Chiba H. Cell adhesion signals regulate the nuclear receptor activity. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24600-24609. doi: 10.1073/pnas.1913346116. Epub 2019 Nov 18. PMID: 31740618; PMCID: PMC6900646.
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