Human CD59 Protein
Catalog No: CD59-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human CD59 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu26-Asn102 (UniProt accession: P13987-1).
Product Details
- Molecular Aliases
- CD59; 1F5; HRF-20; HRF20; MAC-IP; MACIP; MEM43; MACIF; MIRL; G344; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21
- Protein Length
- Leu26-Asn102
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.73 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD59 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD59 is greater than 95% as determined by SEC-HPLC.
Background
CD59 regulates complement activation cascade at the final step, inhibiting formation of membrane attack complex (MAC). This protein, being anchored to the cell membrane via glycosyl phosphatidyl inositol (GPI), is expressed ubiquitously on cells which are in contact with body fluids containing components.
References
- Sugita Y, Masuho Y. CD59: its role in complement regulation and potential for therapeutic use. Immunotechnology. 1995 Dec;1(3-4):157-68. doi: 10.1016/1380-2933(95)00018-6. PMID: 9373344.
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