Biotinylated Human M-CSF R/CSF1R/CD115 Protein
Catalog No: CSF-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human M-CSF R/CSF1R/CD115 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile20-Glu512 (UniProt accession: P07333-1).
Product Details
- Molecular Aliases
- CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
- Protein Length
- Ile20-Glu512
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human M-CSF R is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human M-CSF R, His Tag at 0.5μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Biotinylated Human M-CSF R, His-Avi tag immobilized on CM5 Chip can bind Human M-CSF, No Tag with an affinity constant of 0.81 μM as determined in SPR assay (Biacore T200).
Background
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
References
- Cannarile M A, Weisser M, Jacob W, et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.
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