Biotinylated Human LILRA3/CD85e Protein
Catalog No: LILRA3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human LILRA3/CD85e Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly24-Glu439 (UniProt accession: Q8N6C8).
Product Details
- Molecular Aliases
- CD85e; HM31; HM43; ILT6; ILT-6; LILRA3; LIR4; LIR-4HM43e3; LIR4HM43e3
- Protein Length
- Gly24-Glu439
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human LILRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRA3 is greater than 95% as determined by SEC-HPLC.
Background
Human LILRA3, also known as Leukocyte Ig-like receptor 4 (LIR-4), CD85 antigen-like family member E, Immunogobulin-like transcript 6 (ILT-6) and monocyte inhibitory receptor HM43/HM31, is a 70 kD Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LILRA3 is 416 amino acids (aa) in length and contains four C2-type Ig-like domains. Unlike other LILR family members, LILRA3 is actively secreted. LILRA3 acts as soluble receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.
References
- Ordó?ez, D, Sánchez, A J, Martínez-Rodríguez, J E, et al. Multiple sclerosis associates with LILRA3 deletion in Spanish patients[J]. Genes & Immunity, 2009, 10(6):579-585.
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