Human GM-CSF R alpha Protein
Catalog No: CSF-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human GM-CSF R alpha Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu23-Gly320 (UniProt accession: P15509-1).
Product Details
- Molecular Aliases
- GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
- Protein Length
- Glu23-Gly320
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.5 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
Immobilized Human GM-CSF R alpha, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 0.18μg/ml determined by ELISA.
Background
The receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF) is composed of an alpha and beta subunit, which together form the high-affinity receptor. The alpha subunit by itself binds ligand at low affinity, whereas the isolated beta subunit does not bind GM-CSF. It is generally believed that the high-affinity receptor is responsible for the multiple functions of GM-CSF and that the isolated alpha subunit (GMR alpha) does not transduce a signal.
References
- Ding DX, Rivas CI, Heaney ML, Raines MA, Vera JC, Golde DW. The alpha subunit of the human granulocyte-macrophage colony-stimulating factor receptor signals for glucose transport via a phosphorylation-independent pathway. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2537-41. doi: 10.1073/pnas.91.7.2537. PMID: 8146150; PMCID: PMC43404.
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