Biotinylated Human FGF1 Protein
Catalog No: FGF1-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGF1 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Phe16-Asp155 (UniProt accession: P05230-1).
Product Details
- Molecular Aliases
- FGF-1; aFGF; ECGF; HBGF-1; FGFA; FGF1; HBGF1; Fibroblast growth factor-1; Fibroblast growth factor 1
- Protein Length
- Phe16-Asp155
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.74 kDa. Due to glycosylation, the protein migrates to 20-24 kDa based on Tris-Bis PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).
Background
Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.
References
- Jamal SB, Hockman D. FGF1. Differentiation. 2024 Sep-Oct;139:100802. doi: 10.1016/j.diff.2024.100802. Epub 2024 Jul 22. PMID: 39074995.
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