Biotinylated Human DPPIV/CD26 Protein
Catalog No: DPPIV-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human DPPIV/CD26 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Asn29-Pro766 (UniProt accession: P27487).
Product Details
- Molecular Aliases
- ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5; EC3.4.14.5
- Protein Length
- Asn29-Pro766
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 88.27 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Human DPPIV is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human DPPIV, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. (QC Test)
Background
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.
References
- Enz N, Vliegen G, De Meester I, Jungraithmayr W. DPPIV/CD26 - a potential biomarker and target for cancer therapy. Pharmacol Ther. 2019 Jun;198:135-159. doi: 10.1016/j.pharmthera.2019.02.015. Epub 2019 Feb 26. PMID: 30822465.
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