Biotinylated Human Axl Protein (Primary Amine Labeling)
Catalog No: AXL-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Axl Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala26-Pro449 (UniProt accession: P30530-1).
Product Details
- Molecular Aliases
- Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1;EC2.7.10; EC2.7.10.1
- Protein Length
- Ala26-Pro449
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 72.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Axl on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Axl is greater than 95% as determined by SEC-HPLC.
Immobilized Human GAS6, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).
Immobilized Anti-Axl Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.
Background
Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
References
- Zhu C, Wei Y, Wei X. AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications. Mol Cancer. 2019 Nov 4;18(1):153. doi: 10.1186/s12943-019-1090-3. PMID: 31684958; PMCID: PMC6827209.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.