Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein
Catalog No: BAFF-CB002
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Flag, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed in HEK293 cells with a His tag and Flag tag and Avi tag at the N-terminus. It contains amino acid residues Thr141-Leu285 trimer design (UniProt accession: A0A2K5V2X4).
Product Details
- Molecular Aliases
- BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1; TALL1delta BAFF; TALL1Delta4BAFF
- Protein Length
- Thr141-Leu285
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Cynomolgus BAFF (Trimer), His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve Anti-BAFF Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Immobilized Cynomolgus/Rhesus macaque BAFFR, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus BAFF Trimer, His Avi tag with the EC50 of 0.14μg/ml determined by ELISA.
Biotinylated Cynomolgus BAFF (Trimer), His Tag captured on CM5 Chip via streptavidin can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.40 µM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Cynomolgus BAFF (Trimer), His-Avi-Flag Tag on Streptavidin-Biosensor, can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.69 µM as determined in BLI assay (Gator).
Loaded Human BAFFR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus BAFF (Trimer), His-Avi-Flag Tag with an affinity constant of 0.68 nM as determined in BLI assay.
Background
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene. BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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