Human ACVR1B/Activin RIB Protein
Catalog No: ACVR1B-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human ACVR1B/Activin RIB Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser24-Glu126 (UniProt accession: P36896-1).
Product Details
- Molecular Aliases
- ACVR1B; ACTR-IB; ALK 4; ALK-4; SKR2; ACVRLK4; ALK4; Activin R1B
- Protein Length
- Ser24-Glu126
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 37.36 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human ACVR1B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ACVR1B is greater than 95% as determined by SEC-HPLC.
Loaded Human ACVR1B, hFc Tag on ProA-Biosensor can bind Human TDGF1, His Tag with an affinity constant of 0.13 µM as determined in BLI assay. (QC Test)
Human ACVR1B, hFc Tag captured on CM5 Chip via Protein A can bind Human TDGF1, His Tag with an affinity constant of 0.16 μM as determined in SPR assay (Biacore T200).
Human ACVR1B, hFc Tag captured on CM5 Chip via Protein A can bind Human Activin A, No Tag with an affinity constant of 11.57 nM as determined in SPR assay (Biacore T200).
Background
Activin A receptor type I (ACVR1) encodes for a bone morphogenetic protein type I receptor of the TGFβ receptor superfamily. It is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation. Moreover, ACVR1 has been extensively studied for its causal role in fibrodysplasia ossificans progressiva (FOP), a rare genetic disorder characterised by progressive heterotopic ossification. ACVR1 is linked to different pathologies, including cardiac malformations and alterations in the reproductive system.
References
- Valer JA, Sánchez-de-Diego C, Pimenta-Lopes C, Rosa JL, Ventura F. ACVR1 Function in Health and Disease. Cells. 2019 Oct 31;8(11):1366. doi: 10.3390/cells8111366. PMID: 31683698; PMCID: PMC6912516.
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