Biotinylated Human ASGR1 Protein
Catalog No: ASGR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human ASGR1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gln62-Leu291 (UniProt accession: P07306-1).
Product Details
- Molecular Aliases
- ASGP-R 1; ASGPR 1; Hepatic lectin 1; HL-1; mHL-1; Asgr1; Asgr-1;; ASGPR1; HL1; mHL1
- Protein Length
- Gln62-Leu291
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 29.30 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ASGR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ASGR1 is greater than 95% as determined by SEC-HPLC.
Background
ASGR1 is a liver-specific surface marker that is exclusively expressed in liver and mediates internalization and lysosomal degradation of blood asialoglycoproteins.
References
- Wang JQ, Li LL, Hu A, Deng G, Wei J, Li YF, Liu YB, Lu XY, Qiu ZP, Shi XJ, Zhao X, Luo J, Song BL. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion. Nature. 2022 Aug;608(7922):413-420. doi: 10.1038/s41586-022-05006-3. Epub 2022 Aug 3. PMID: 35922515.
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