Biotinylated Human BAFFR/TNFRSF13C Protein
Catalog No: BAFFR-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser7-Ala71 (UniProt accession: Q96RJ3-1).
Product Details
- Molecular Aliases
- CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
- Protein Length
- Ser7-Ala71
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human BAFFR is greater than 90% as determined by SEC-HPLC.
Immobilized Human BAFF Trimer, hFc Tag (Cat. BAF-HM213) at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Avi tag with the EC50 of 30.2ng/ml determined by ELISA. (QC Test)
Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.
Loaded Biotinylated Human BAFFR, His-Avi tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay.
Loaded Biotinylated Human BAFFR, His-Avi tag on Anti-His-Biosensor can bind Anti-BAFFR Ab., hFc Tag with an affinity constant of 18.5 pM as determined in BLI assay.
Background
BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells
References
- Smulski CR, Eibel H. BAFF and BAFF-Receptor in B Cell Selection and Survival. Front Immunol. 2018 Oct 8;9:2285. doi: 10.3389/fimmu.2018.02285. PMID: 30349534; PMCID: PMC6186824.
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