Biotinylated Human Her2/ErbB2 Protein
Catalog No: HER2ERBB2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Her2/ErbB2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr23-Thr652 (UniProt accession: P04626-1).
Product Details
- Molecular Aliases
- CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW
- Protein Length
- Thr23-Thr652
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Her2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Her2, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).
Background
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.
References
- Tagliabue E, Balsari A, Campiglio M, et al. HER2 as a target for breast cancer therapy[J]. EXPERT OPINION ON BIOLOGICAL THERAPY, 2010, 10(5):711-724.
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