Biotinylated Human BTN2A1 Protein
Catalog No: BTN2A1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human BTN2A1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln29-Ala248 (UniProt accession: Q7KYR7-1).
Product Details
- Molecular Aliases
- BT2.1; BTF1; BK14H9.1; BTF1BT2.1; DJ3E1.1; FLJ36567
- Protein Length
- Gln29-Ala248
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.47 kDa. Due to glycosylation, the protein migrates to 43-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human BTN2A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BTN2A1 is greater than 95% as determined by SEC-HPLC.
Background
The MHC-encoded butyrophilin, BTN2A1, is a cell surface glycoprotein related to the extended family of B7 costimulatory molecules. BTN2A1 mRNA was expressed in most human tissues, but protein expression was significantly lower in leukocytes.
References
- Malcherek G, Mayr L, Roda-Navarro P, Rhodes D, Miller N, Trowsdale J. The B7 homolog butyrophilin BTN2A1 is a novel ligand for DC-SIGN. J Immunol. 2007 Sep 15;179(6):3804-11. doi: 10.4049/jimmunol.179.6.3804. PMID: 17785817.
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