Human IL-22R alpha 1 Protein
Catalog No: IL22RALPHA1-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-22R alpha 1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues His16-Thr228 (UniProt accession: Q8N6P7).
Product Details
- Molecular Aliases
- IL-22R-alpha-1; IL-22RA1; CRF2-9; IL22R; IL22R1; IL-TIF-R1; zcytoR11; IL22R-alpha-1; IL22RA1
- Protein Length
- His16-Thr228
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-22, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 10.6 ng/ml determined by ELISA (QC Test).
Immobilized Human IL-22R alpha 1, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-22R alpha 1 Antibody, hFc Tag with the EC50 of 19.2 ng/ml determined by ELISA.
Loaded Human IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Human IL-22, His Tag with an affinity constant of 42.60 nM as determined in BLI assay.
Loaded Biotinylated Anti-IL-22RA1 Antibody, hFc-Avi tag on Streptavidin-Biosensor can bind Human IL-22RA1, hFc Tag with an affinity constant of 1.14 nM as determined in BLI assay.
Background
Interleukin (IL)-22 is a member of the IL-10 family of cytokines and represents an important effector molecule of activated Th22, Th1, and Th17 cells, as well as Tc-cell subsets, gammadelta T cells, natural killer (NK), and NKT cells. IL-22 mediates its effects via a heterodimeric transmembrane receptor complex consisting of IL-22R1 and IL-10R2 and subsequent Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathways including Jak1, Tyk2, and STAT3.
References
- Wolk K, Witte E, Witte K, Warszawska K, Sabat R. Biology of interleukin-22. Semin Immunopathol. 2010 Mar;32(1):17-31. doi: 10.1007/s00281-009-0188-x. Epub 2010 Feb 2. PMID: 20127093.
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