Biotinylated Human MASP3 Protein
Catalog No: MASP3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human MASP3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile450-Arg728 (UniProt accession: P48740-2).
Product Details
- Molecular Aliases
- MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5;; MASP3
- Protein Length
- Ile450-Arg728
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 33.46 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MASP3 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human MASP3, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. (QC Test)
Background
Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system.
References
- Mu L, Yin X, Wu H, Han K, Wu L, Ding M, Bian X, Li B, Fu S, Liang F, Guo Z, Ye J. Expression and functional characterization of a mannose-binding lectin-associated serine protease-1 (MASP-1) from Nile tilapia (Oreochromis niloticus) in host defense against bacterial infection. Fish Shellfish Immunol. 2019 Aug;91:68-77. doi: 10.1016/j.fsi.2019.05.014. Epub 2019 May 13. PMID: 31096060.
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