Biotinylated Human IL-17R alpha/CD217 Protein
Catalog No: CD217-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Leu33-Trp320 (UniProt accession: Q96F46-1).
Product Details
- Molecular Aliases
- CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα;IL17RA; IL17R-alpha;IL17R alpha
- Protein Length
- Leu33-Trp320
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17R alpha is greater than 90% as determined by SEC-HPLC.
Immobilized Human IL-17A, His Tag (Cat. ILA-HM117) at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi tag with the EC50 of 0.24μg/ml determined by ELISA (QC Test).
Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94μg/ml determined by ELISA.
Immobilized Human IL-17F, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76μg/ml determined by ELISA.
Loaded Biotinylated Human IL-17R alpha, hFc-Avi tag on Steptavidin-Biosensor can bind Human IL-17A, His Tag with an affinity constant of 5.73 nM as determined in BLI assay.
Background
Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.
References
- Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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