Biotinylated Human VEGF R1/FLT-1 Protein
Catalog No: VEGFR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human VEGF R1/FLT-1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser27-Asn756 (UniProt accession: P17948-1).
Product Details
- Molecular Aliases
- FLT; FLT1; Flt-1; FRT; VEGF R1; VEGFR1
- Protein Length
- Ser27-Asn756
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human VEGF R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test).
Immobilized Human VEGF121, His Tag at 2μg/ml (100ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8ng/ml determined by ELISA.
Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 48ng/ml determined by ELISA.
Background
VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs).yrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells.
References
- Fischer C, Mazzone M, Jonckx B, et al. FLT1 and its ligands VEGFB and PlGF: drug targets for anti-angiogenic therapy?[J]. NATURE REVIEWS CANCER, 2008, 8(12):942-956.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.