Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling)
Catalog No: TNFSF15-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human TNFSF15 Trimer Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Asp91-Leu251 trimer design (UniProt accession: O95150-1).
Product Details
- Molecular Aliases
- TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; VEGI251
- Protein Length
- Asp91-Leu251
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse DR3, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.22 μg/ml determined by ELISA (QC Test).
Immobilized Human DR3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TNFSF15 Trimer, His Tag with the EC50 of 0.34μg/ml determined by ELISA.
Immobilized Biotinylated Human TNFSF15 Trimer, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human DcR3, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human TNFSF15 Trimer, His Tag with an affinity constant of 3.64 nM as determined in SPR assay (Biacore T200).
Background
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
References
- Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019;10:583. Published 2019 Mar 27. doi:10.3389/fimmu.2019.00583
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