Biotinylated Human VEGF-C/Flt4-L Protein
Catalog No: VEGFC-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr103-Arg227 (UniProt accession: Q6FH59).
Product Details
- Molecular Aliases
- VEGFC; VEGFc; Flt4-L; VRP; VEGF-C;Flt4L
- Protein Length
- Thr103-Arg227
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 50mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.
Immobilized Biotinylated Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Background
the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.
References
- Lim L, Bui H, Farrelly O, Yang J, Li L, Enis D, Ma W, Chen M, Oliver G, Welsh JD, Kahn ML. Hemostasis stimulates lymphangiogenesis through release and activation of VEGFC. Blood. 2019 Nov 14;134(20):1764-1775. doi: 10.1182/blood.2019001736. PMID: 31562136; PMCID: PMC6856989.
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