Biotinylated Human SERPINF2/A2AP Protein
Catalog No: SERPINF2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human SERPINF2/A2AP Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Met28-Lys491 (UniProt accession: P08697-1).
Product Details
- Molecular Aliases
- Alpha-2-AP; AAP; PLI; SERPINF2; A2AP; ALPHA-2-PI; API; Serpin F2;Alpha-2AP; ALPHA-2PI
- Protein Length
- Met28-Lys491
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 54.62 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human SERPINF2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SERPINF2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human SERPINF2, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. (QC Test)
Background
Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP; SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.
References
- Burnouf T, Goubran HA, Radosevich M, Sayed MA, Gorgy G, El-Ekiaby M. Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 2007 Oct;35(4):349-53. doi: 10.1016/j.biologicals.2007.03.002. Epub 2007 Jul 26. PMID: 17656111.
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