Biotinylated Human PSMA/FOLH1 Protein (active dimer)
Catalog No: PSMA-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human PSMA/FOLH1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Lys44-Ala750 (UniProt accession: Q04609-1).
Product Details
- Molecular Aliases
- GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
- Protein Length
- Lys44-Ala750
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 82.43 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 50mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Human PSMA is greater than 85% as determined by SEC-HPLC.
The purity of Biotinylated Human PSMA is greater than 85% and the molecular weight of this protein is around 180-220 kDa as determined by SEC-MALS.
Immobilized Biotinylated Human PSMA, His-Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA. (QC Test)
Background
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
References
- Song C, Francesca Z, Williams D G, et al. Antitumor Activity of MEDI3726 (ADCT-401), a Pyrrolobenzodiazepine Antibody-drug Conjugate Targeting PSMA, in Pre-clinical Models of Prostate Cancer[J]. Molecular Cancer Therapeutics, 2018:molcanther.0982.2017-.
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