Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein
Catalog No: GARP-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus of GARP. It contains amino acid residues Ala18-Leu628(GARP) and Leu30-Ser390(Latent TGF beta 1) (accession: A0A2K5X2X9(GARP) & A0A2K5TJB2(Latent TGF beta 1)).
Product Details
- Molecular Aliases
- LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1; TGF-b-1; TGF-b1; LRRC32&TGF-b 1
- Protein Length
- Ala18-Leu628
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus ITGAV&ITGB6, His Tag (Cat. ITG-CM1V6) at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His-Avi tag with the EC50 of 0.11μg/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Background
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
References
- Stockis J, Dedobbeleer O, Lucas S. Role of GARP in the activation of latent TGF-β1. Mol Biosyst. 2017 Sep 26;13(10):1925-1935.
- Metelli A, Salem M, Wallace CH, Wu BX, Li A, Li X, Li Z. Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer. J Hematol Oncol. 2018 Feb 20;11(1):24.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.