Biotinylated Human GCGR/Glucagon receptor Nanodisc
Catalog No: GCGR-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Phe477 (UniProt accession: P47871).
Product Details
- Molecular Aliases
- Glucagon receptor; GL-R; GCGR; Glucagon R
- Protein Length
- Met1-Phe477
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 66.9 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).
Biotinylated Human GCGR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 5.02 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human GCGR Nanodisc, His Tag on Streptavidin -Biosensor can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 8.61 nM as determined in BLI assay.
Background
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.
References
- Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D'Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.
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