Biotinylated Human IL-17A&F Protein
Catalog No: IL17AF-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- HA
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a HA tag at the N-terminus and subunit IL-17F is fused with a His tag and a Avi tag at the N-terminus. It contains amino acid residues Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F) (accession: Q16552-1(IL-17A) & Q96PD4(IL-17F)).
Product Details
- Molecular Aliases
- IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F;IL17A; CTLA8; IL17; IL17F; ML1;Interleukin-17; Interleukin 17; Interleukin-24; Interleukin 24
- Protein Length
- Gly24-Ala155
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.35 kDa (IL-17A) and 17.81 kDa (IL-17F). Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17A&F is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-17A&F, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 16.9ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human IL-17A&F, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Background
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
References
- Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.
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