Human CTLA-4/CD152 Protein
Catalog No: CD152-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CTLA-4/CD152 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys36-Asp161 (UniProt accession: P16410-1).
Product Details
- Molecular Aliases
- CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
- Protein Length
- Lys36-Asp161
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Immobilized Human B7-1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Immobilized Human B7-2, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Loaded Human CTLA-4, hFc Tag on ProA-Biosensor can bind Human B7-1, His Tag with an affinity constant of 0.21 µM as determined in BLI assay.
Loaded Human CTLA-4, hFc Tag on ProA-Biosensor can bind Human B7-2, His Tag with an affinity constant of 0.15 µM as determined in BLI assay.
Background
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
References
- Merwe V D, P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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