Cynomolgus BDCA-2 Protein
Catalog No: BDCA2-CF002
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- an mFc (IgG2a)
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus BDCA-2 Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the N-terminus. It contains amino acid residues Tyr48-Ile212 (UniProt accession: A0A2K5UWP4).
Product Details
- Molecular Aliases
- BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
- Protein Length
- Tyr48-Ile212
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.00 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus BDCA-2 is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus BDCA-2, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA (QC Test).
Loaded Anti-BDCA-2 Antibody, hFc Tag on HFC-Biosensor can bind Cynomolgus BDCA-2, mFc Tag with an affinity constant of 0.32 nM as determined in BLI assay.
Background
BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.
References
- Jégouzo SA, Feinberg H, Dungarwalla T, Drickamer K, Weis WI, Taylor ME. A Novel Mechanism for Binding of Galactose-terminated Glycans by the C-type Carbohydrate Recognition Domain in Blood Dendritic Cell Antigen 2. J Biol Chem. 2015 Jul 3;290(27):16759-71. doi: 10.1074/jbc.M115.660613. Epub 2015 May 20. PMID: 25995448; PMCID: PMC4505424.
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