Biotinylated Human MICA Protein
Catalog No: MICA-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human MICA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu24-Gln308 (UniProt accession: Q96QC4).
Product Details
- Molecular Aliases
- FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250; DAMA345G11.2
- Protein Length
- Glu24-Gln308
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human MICA, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
Immobilized Anti-MICA Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7μg/ml determined by ELISA.
Background
TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
References
- Friese M A, Platten M, Lutz S Z, et al. MICA/NKG2D-mediated immunogene therapy of experimental gliomas.[J]. Cancer Research, 2003, 63(24):8996-9006.
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