Human uPAR/PLAUR Protein
Catalog No: PLAUR-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human uPAR/PLAUR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu23-Gly305 (UniProt accession: Q03405-1).
Product Details
- Molecular Aliases
- U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
- Protein Length
- Leu23-Gly305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human uPAR is greater than 95% as determined by SEC-HPLC.
Immobilized Human PLAU, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
Background
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
References
- Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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