Biotinylated Human RGM-C Protein
Catalog No: RGMC-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human RGM-C Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Gln36-Asp400 (UniProt accession: Q6ZVN8-1).
Product Details
- Molecular Aliases
- Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
- Protein Length
- Gln36-Asp400
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.67 kDa. Due to autocatalytic cleavage, the protein migrates to 50-60 kDa (mature), 30-40 kDa (C-terminus chain) and 20-25 kDa (N-terminus chain) based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human RGM-C is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human RGM-C, Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Background
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
References
- Oldekamp J, Krämer N, Alvarez-Bolado G, Skutella T. Expression pattern of the repulsive guidance molecules RGM A, B and C during mouse development. Gene Expr Patterns. 2004 May;4(3):283-8. doi: 10.1016/j.modgep.2003.11.008. PMID: 15053976.
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