Biotinylated Human MRGPRX2 Nanodisc
Catalog No: MRGPRX2-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human MRGPRX2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val330 (UniProt accession: Q96LB1).
Product Details
- Molecular Aliases
- MRGX2
- Protein Length
- Met1-Val330
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.1 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human MRGPRX2 Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MRGPRX2 Antibody, mFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human MRGPRX2 Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-MRGPRX2 Antibody, mFc Tag with an affinity constant of 0.25 nM as determined in BLI assay.
Background
As receptor for multiple drugs, MRGPRX2 is crucial to drug-induced hypersensitivity. However, not only drugs, but also endogenous mediators like neuropeptides and host defense peptides activate MRGPRX2, suggesting its broad impact in cutaneous pathophysiology.
References
- Wang Z, Babina M. MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis? Exp Dermatol. 2020 Nov;29(11):1104-1111. doi: 10.1111/exd.14182. Epub 2020 Sep 17. PMID: 32866307.
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