Biotinylated SARS-COV-2 Spike S1 Protein
Catalog No: SARS-HB004
- Species
- SARS-COV-2
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated SARS-CoV-2 Spike S1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln14-Arg683 (accession: QHD43416.1).
Product Details
- Molecular Aliases
- S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
- Protein Length
- Gln14-Arg683
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated SARS-CoV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated SARS-CoV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated SARS-CoV-2 Spike S1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
References
- Belouzard S, Chu V C, Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
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