Biotinylated Human VEGF121 Protein
Catalog No: VEGF121-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human VEGF121 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala27-Arg147 (UniProt accession: P15692-9).
Product Details
- Molecular Aliases
- VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
- Protein Length
- Ala27-Arg147
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human VEGF R1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.2ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human VEGF121, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Background
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
References
- Rosen, L. S. VEGF-Targeted Therapy: Therapeutic Potential and Recent Advances[J]. The Oncologist, 2005, 10(6):382-391.
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