Cynomolgus CRTAM Protein
Catalog No: CRTAM-CF001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus CRTAM Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser18-Gly287 (accession: XP_005580021.1).
Product Details
- Molecular Aliases
- CD355 antigen; CD355; CRTAM
- Protein Length
- Ser18-Gly287
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 56.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus CRTAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CRTAM is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus CRTAM, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CRTAM Antibody, hFc Tag with the EC50 of 73.9ng/ml determined by ELISA.
Background
Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile; therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.
References
- Takeuchi A, Itoh Y, Takumi A, Ishihara C, Arase N, Yokosuka T, Koseki H, Yamasaki S, Takai Y, Miyoshi J, Ogasawara K, Saito T. CRTAM confers late-stage activation of CD8 T cells to regulate retention within lymph node. J Immunol. 2009 Oct 1;183(7):4220-8. doi: 10.4049/jimmunol.0901248. Epub 2009 Sep
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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