Biotinylated Human SEZ6 Protein
Catalog No: SEZ6-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human SEZ6 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu20-His925 (UniProt accession: Q53EL9-1).
Product Details
- Molecular Aliases
- SEZ-6; SEZ6; BSRPC; BSRP-C
- Protein Length
- Leu20-His925
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human SEZ6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human SEZ6, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
Background
Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
References
- Roitman M, Edgington-Mitchell LE, Mangum J, Ziogas J, Adamides AA, Myles P, Choo-Bunnett H, Bunnett NW, Gunnersen JM. Sez6 levels are elevated in cerebrospinal fluid of patients with inflammatory pain-associated conditions. Pain Rep. 2019 Mar 25;4(2):e719. doi: 10.1097/PR9.0000000000000719. PMID: 31041421; PMCID: PMC6455686.
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