Mouse BAFFR/TNFRSF13C Protein
Catalog No: BAFFR-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Mouse BAFFR/TNFRSF13C Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser10-Ala71 (UniProt accession: Q9D8D0-1).
Product Details
- Molecular Aliases
- CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
- Protein Length
- Ser10-Ala71
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse BAFFR is greater than 95% as determined by SEC-HPLC.
Immobilized Human BAFF (Trimer), His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse BAFFR, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC test).
Immobilized Mouse BAFF, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse BAFFR, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Loaded Mouse BAFFR, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 2.02 nM as determined in BLI assay.
Background
BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor; a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells
References
- Smulski CR, Eibel H. BAFF and BAFF-Receptor in B Cell Selection and Survival. Front Immunol. 2018 Oct 8;9:2285. doi: 10.3389/fimmu.2018.02285. PMID: 30349534; PMCID: PMC6186824. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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