Biotinylated Mouse IL-22 Protein
Catalog No: IL22-MB001
- Species
- Mouse
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse IL-22 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Leu34-Val179 (UniProt accession: Q9JJY9).
Product Details
- Molecular Aliases
- Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18;IL22; TIFIL23;IL 22; Interleukin 22
- Protein Length
- Leu34-Val179
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Mouse IL-22, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).
Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 μM as determined in SPR assay (Biacore T200).
Background
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
References
- Keir M, Yi Y, Lu T, Ghilardi N. The role of IL-22 in intestinal health and disease. J Exp Med. 2020 Feb 13;217(3):e20192195. doi: 10.1084/jem.20192195. PMID: 32997932; PMCID: PMC7062536.
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