Human IL-2 R beta&IL-2 R gamma Protein
Catalog No: IL2RBIL2RG-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus of IL-2 R beta and IL-2 R gamma. It contains amino acid residues Ala27-Thr240 (IL-2 R beta) and Leu23-Ala262 (IL-2 R gamma) (accession: P14784(IL-2 R beta) & P31785-1(IL-2 R gamma)).
Product Details
- Molecular Aliases
- IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ;IL2RB&IL2RG; IL2RG; IL2R subunit gamma; IL2R subunit beta; IL2RB; IL2R beta&IL2R gamma; IL2R subunit β; IL2R β&IL2R gamma; IL2Rγ; IL2R γ;IL-2R subunit b; IL2R subunit b; IL-2 R b&IL-2 R gamma; IL2R b&IL2R gamma; IL-2R gamma; IL2R gamma; IL-2R g; IL2R g; IL-2 Rg; IL-2 R gamma; IL-2 R g
- Protein Length
- Ala27-Thr240
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51.3 kDa (IL-2 R beta) and 54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa and 80-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human IL-15RA&IL-15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Background
Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.
References
- Dejjuy D, Dechsukhum C, Pattanapanyasat K, Noulsri E, Dissen GA, Leeanansaksiri W. Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells. Biomed Res Int. 2020 Oct 14;2020:7851414. doi: 10.1155/2020/7851414. PMID: 33110919; PMCID: PMC7582064.
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