Human CD228/MFI2 Protein
Catalog No: CD228-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD228/MFI2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag tag at the C-terminus. It contains amino acid residues Gly20-Cys709 (UniProt accession: P08582-1).
Product Details
- Molecular Aliases
- Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
- Protein Length
- Gly20-Cys709
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 101.45 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD228 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD228, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA. (QC Test)
Background
Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.
References
- Bonhoure A, Henry L, Morille M, Aissaoui N, Bellot G, Stoebner PE, Vidal M. Melanotransferrin is efficiently sorted on the surface of exosomes secreted by melanoma cells. Melanoma Res. 2021 Aug 1;31(4):338-351. doi: 10.1097/CMR.0000000000000741. PMID: 33965973.
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