Biotinylated Human IL-3 Protein
Catalog No: IL3-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-3 Protein is expressed in E. coli with an Avi tag at the N-terminus. It contains amino acid residues Ala20-Phe152 (UniProt accession: P08700).
Product Details
- Molecular Aliases
- IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF;IL3MGC79398;Interleukin 3; IL 3
- Protein Length
- Ala20-Phe152
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-3, Avi tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test).
Background
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
References
- Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.
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