Biotinylated Human EPO R/Erythropoietin R Protein
Catalog No: EPO-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human EPO R/Erythropoietin R Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Ala25-Pro250 (UniProt accession: P19235-1).
Product Details
- Molecular Aliases
- EPO-R; Erythropoietin R; EPO R; EPOR
- Protein Length
- Ala25-Pro250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.58 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human EPO R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human EPO R is greater than 90% as determined by SEC-HPLC.
Immobilized Human EPO, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70μg/ml determined by ELISA.
Background
The erythropoietin (epo) receptor is a member of the cytokine receptor family. It is expressed almost exclusively on erythroid precursor cells and controls the development of red blood cells. The epo receptor has no intrinsic kinase activity, but binds intracellular tyrosine kinases to elicit its signals. Alterations in the transmission of the signalling cascade lead to clinically abnormal red blood cell production.
References
- Tilbrook PA, Klinken SP. The erythropoietin receptor. Int J Biochem Cell Biol. 1999 Oct;31(10):1001-5. doi: 10.1016/s1357-2725(99)00071-0. PMID: 10582333.
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