Biotinylated Human NPR1/NPRA Protein (active dimer)
Catalog No: NPR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NPR1/NPRA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly33-Glu473 (accession: NP_000897.3).
Product Details
- Molecular Aliases
- ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
- Protein Length
- Gly33-Glu473
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NPR1 is greater than 90% as determined by SEC-HPLC.
The purity of Biotinylated Human NPR1 is greater than 90% and the molecular weight of this protein is around 110-120 kDa as determined by SEC-MALS.
Immobilized Biotinylated Human NPR1, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Anti-NPR1 Ab.2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human NPR1, His-Avi tag on Streptavidin-Biosensor can bind Anti-NPR1 Ab.2, hFc Tag with an affinity constant of 1.35 nM as determined in BLI assay.
Background
NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
References
- Tokudome T, Otani K, Mao Y, Jensen LJ, Arai Y, Miyazaki T, Sonobe T, Pearson JT, Osaki T, Minamino N, Ishida J, Fukamizu A, Kawakami H, Onozuka D, Nishimura K, Miyazato M, Nishimura H. Endothelial Natriuretic Peptide Receptor 1 Play Crucial Role for Acute and Chronic Blood Pressure Regulation by Atrial Natriuretic Peptide. Hypertension. 2022 Jul;79(7):1409-1422. doi: 10.1161/HYPERTENSIONAHA.121.18114. Epub 2022 May 9. PMID: 35534926.
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