Biotinylated Human NKG2D/CD314 Protein
Catalog No: NKG2D-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi, His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NKG2D/CD314 Protein is expressed in HEK293 cells with an Avi tag and His tag at the N-terminus. It contains amino acid residues Phe78-Val216 (UniProt accession: P26718).
Product Details
- Molecular Aliases
- CD314; D12S2489E; KLR; NKG2-D; NKG2D
- Protein Length
- Phe78-Val216
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2D is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human NKG2D, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Immobilized Anti-NKG2D Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKG2D, His-Avi tag with the EC50 of 0.39μg/ml determined by ELISA.
Background
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
References
- Spear P, Barber A, Rynda-Apple A, et al. NKG2D CAR T-cell therapy inhibits the growth of NKG2D ligand heterogeneous tumors[J]. Immunology and Cell Biology, 2013, 91(6):435-440.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.