Biotinylated Human PSGL-1 Protein
Catalog No: PSGL1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human PSGL-1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln42-Cys320 (UniProt accession: Q14242-1).
Product Details
- Molecular Aliases
- CLA; PSGL-1; PSGL1; CD162; SELPLG; Selectin P ligand; Selp1; Selpl
- Protein Length
- Gln42-Cys320
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.74 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human PSGL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PSGL-1 is greater than 95% as determined by SEC-HPLC.
Background
P-selectin glycoprotein ligand-1 (PSGL-1) has long been studied as an adhesion molecule involved in immune cell trafficking and is recognized as a regulator of many facets of immune responses by myeloid cells. PSGL-1 also regulates T cell migration during homeostasis and inflammatory settings.
References
- Tinoco R, Otero DC, Takahashi AA, Bradley LM. PSGL-1: A New Player in the Immune Checkpoint Landscape. Trends Immunol. 2017 May;38(5):323-335. doi: 10.1016/j.it.2017.02.002. Epub 2017 Mar 2. PMID: 28262471; PMCID: PMC5411281.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.