Biotinylated Human Oncostatin M/OSM Protein
Catalog No: OSM-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Oncostatin M/OSM Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala26-Arg221 (UniProt accession: P13725).
Product Details
- Molecular Aliases
- Oncostatin M; OSM; MGC20461
- Protein Length
- Ala26-Arg221
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Oncostatin M on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Oncostatin M is greater than 95% as determined by SEC-HPLC.
Human LIF R, His Tag immobilized on CM5 Chip can bind Biotinylated Human Oncostatin M, His Tag with an affinity constant of 0.94 μM as determined in SPR assay (Biacore T200).
Background
OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
References
- Junk D J, Bryson B L, Smigiel J M, et al. Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling[J]. Oncogene, 2017.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.