Biotinylated Human OSMR beta Protein
Catalog No: OSMR-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human OSMR beta Protein is expressed in HEK293 cells with an Avi tag at the C-terminus. It contains amino acid residues Glu28-Met740 (UniProt accession: Q99650-1).
Product Details
- Molecular Aliases
- IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1; IL-31R subunit β; IL-31R-β; OSMR β; IL31R subunit beta; IL31R-beta; IL31RB; IL31R subunit β; IL31R-β; IL-31R subunit b; IL31R subunit b; IL-31R-b; IL31R-b; OSMR b
- Protein Length
- Glu28-Met740
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 82.93 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human OSMR beta is greater than 85% as determined by SEC-HPLC.
Immobilized Biotinylated Human OSMR beta, Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Background
OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
References
- Sharanek A, Burban A, Laaper M, Heckel E, Joyal JS, Soleimani VD, Jahani-Asl A. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun. 2020 Aug 17;11(1):4116. doi: 10.1038/s41467-020-17885-z. PMID: 32807793; PMCID: PMC7431428.
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