Biotinylated Human TFPI Protein
Catalog No: TFPI-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human TFPI Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp29-Lys282 (UniProt accession: P10646-1).
Product Details
- Molecular Aliases
- LACI; TFPI1; EPI; TFI; convertin; TFPI
- Protein Length
- Asp29-Lys282
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 32.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human TFPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development.
References
- Ali HO, Stavik B, Dørum E, Iversen N, Sandset PM, Skretting G. Oestrogen induced downregulation of TFPI expression is mediated by ERα. Thromb Res. 2014;134(1):138-143. doi:10.1016/j.thromres.2014.04.004
Have questions about this product?
Our technical team can help with product selection, application questions and order support.