Biotinylated Mouse uPAR/PLAUR Protein
Catalog No: PLAUR-MB003
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse uPAR/PLAUR Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu24-Gly298 (UniProt accession: P35456-1).
Product Details
- Molecular Aliases
- U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
- Protein Length
- Leu24-Gly298
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse uPAR is greater than 95% as determined by SEC-HPLC.
Human PLAU, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse uPAR, His Tag with an affinity constant of 418.50 nM as determined in SPR assay (Biacore T200).
Background
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen. Regulation of the activity of u-PA is also mediated by u-PAR.
References
- Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID: 9122717.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.