Mouse CD43 Protein
Catalog No: CD43-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Mouse CD43 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Asp20-Gly248 (UniProt accession: P15702).
Product Details
- Molecular Aliases
- Leukosialin; CD43; Ly-48; A630014B01Rik; Galgp; GPL115; LSN; Sialophorin; SPN;Ly48
- Protein Length
- Asp20-Gly248
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.30 kDa. Due to glycosylation, the protein migrates to 75-120 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse CD43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD43 is greater than 95% as determined by SEC-HPLC.
Background
CD43 is a large transmembrane protein involved in T cell activation. Previous studies of CD43-/- mice in viral models have demonstrated a role for CD43 in Th1/Th2 skewing, activation of Foxp3 Treg, and T cell apoptosis. CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions.
References
- Alisson-Silva F, Mantuano NR, Lopes AL, Vasconcelos-Dos-Santos A, Vale AM, Costa MM, Cannon JL, Oliveira AC, Todeschini AR. CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection. Sci Rep. 2019 Jun 13;9(1):8628. doi: 10.1038/s41598-019-45138-7. PMID: 31197200; PMCID: PMC6565700. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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