Biotinylated Human CD117/c-kit Protein
Catalog No: CD117-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD117/c-kit Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln26-Pro520 (UniProt accession: P10721-2).
Product Details
- Molecular Aliases
- SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; EC2.7.10; EC2.7.10.1
- Protein Length
- Gln26-Pro520
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 58.60 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD117 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD117, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Immobilized Human SCF, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8μg/ml determined by ELISA.
Background
The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra, PDGF-Rb), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor.
References
- Escribano L, Ocqueteau M, Almeida J, Orfao A, San Miguel JF. Expression of the c-kit (CD117) molecule in normal and malignant hematopoiesis. Leuk Lymphoma. 1998 Aug;30(5-6):459-66. doi: 10.3109/10428199809057558. PMID: 9711908.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.